Schematic illustration of the CRISPR-PITA, targeting of scFv-LANA or any other protein of interest to the telomeres via dCas9. Then, the recruitment of other nuclear proteins to these dots is evaluated via immunostaining.

Lavi I, Bhattacharya S, Awase A, Orgil O, Avital N, Journo G, Gurevich V, Shamay M. Unidirectional recruitment between MeCP2 and KSHV-encoded LANA revealed by CRISPR/Cas9 recruitment assay. PLoS Pathog. 2025 Mar 10;21(3):e1012972. doi: 10.1371/journal.ppat.1012972